Fig. 3: Modulation of the inflammatory response by Ifnar1 signaling during necroptosis stimulation. | Cell Death & Disease

Fig. 3: Modulation of the inflammatory response by Ifnar1 signaling during necroptosis stimulation.

From: Regulation of Zfp36 by ISGF3 and MK2 restricts the expression of inflammatory cytokines during necroptosis stimulation

Fig. 3

WT and Ifnar1−/− BMDMs were treated with LPS (1 ng/mL) in the presence of zVAD-fmk (50 µM) for 6 h and differential gene expression and GSEA was performed on microarray data comparing WT BMDMs treated with LPS+zVAD with Ifnar1−/− BMDMs treated with LPS+zVAD (A–D). E Expression levels of TNFα, IFNβ, IL-6, and IL-10 were measured in the supernatants collected after treating WT and Ifnar1−/− BMDMs for 7 h with LPS (1 ng/mL) and EMR (10 µM). F, G Western blot analysis was performed in cell extracts collected from WT and Ifnar1−/− BMDMs at different time intervals following treatment with LPS (1 ng/mL) and EMR (10 µM). Each experiment was repeated at least three times. (****P < 0.0001).

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