Fig. 7: Ifnar1-signaling promotes the degradation of TNFα mRNA. | Cell Death & Disease

Fig. 7: Ifnar1-signaling promotes the degradation of TNFα mRNA.

From: Regulation of Zfp36 by ISGF3 and MK2 restricts the expression of inflammatory cytokines during necroptosis stimulation

Fig. 7

A–D Expression of Il-12, Cxcl11, and Ifnb1 mRNA were measured by qRT-PCR analysis at 3 h following treatment of WT and Ifnar1−/− BMDMs with LPS (1 ng/mL) and EMR (10 µM). E, F Expression of Tnfa mRNA was measured by qRT-PCR analysis at various time intervals post treatment of WT and Ifnar1−/− BMDMs with LPS (1 ng/mL), EMR (10 µM), and IFNβ (10 ng/mL). G, H Expression of TNFα was measured by ELISA in the supernatants collected at different time intervals following treatment of WT and Ifnar1−/− BMDMs with LPS (1 ng/mL), EMR (10 µM), and IFNβ (10 ng/mL), or Actinomycin D (2 µM). Each experiment was at least repeated three times. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).

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