Fig. 2: Pdk4 knockout (Pdk4−/−) mice show reduced choroidal neovascularization (CNV).

A Grading of CNV lesions (0, 1, 2A, and 2B) was conducted in fluorescein angiography images from wild-type (WT) and Pdk4−/− (Pdk4 KO) mice 7 days following CNV induction (n = 5 mice/group). B CNV lesion size was calculated in both groups. **P < 0.01; ***P < 0.001 versus wild-type CNV (n = 34 lesions/group). Chi-square and two-tailed unpaired t-test. C mRNA levels of proinflammatory cytokines, including interleukin-1 beta (Il1b), interleukin-6 (Il6), and monocyte chemoattractant protein-1 (Mcp1), were measured. Data are represented as mean ± SEM. ##P < 0.01; ###P < 0.001 versus wild type; **P < 0.01; ***P < 0.001 versus wild-type CNV (n = 5 mice/group). Scale bar: 100 μm. One-way ANOVA with Tukey’s multiple comparisons test. NV neovascularization.