Fig. 4: TCP1 binds with and stabilises ACSL4 protein to reduce ACSL4 ubiquitination and degradation. | Cell Death & Disease

Fig. 4: TCP1 binds with and stabilises ACSL4 protein to reduce ACSL4 ubiquitination and degradation.

From: TCP1 expression alters the ferroptosis sensitivity of diffuse large B-cell lymphoma subtypes by stabilising ACSL4 and influences patient prognosis

Fig. 4

A Fluorescence microscopic observation of the co-localisation of TCP1 (red) and ACSL4 (green) in TCP1-overexpressing SU-DHL-4 cells. Nuclei were counterstained with DAPI (blue). B, C Verification of the interaction between ACSL4 and TCP1 through co-IP in DB and SU-DHL-4 cells. D Western blot analysis of ACSL4 expression levels in TCP1-knockdown DB and SU-DHL-4 cells in the presence or absence of the proteasome inhibitor MG132 (20 μM). E Detection of TCP1 and ACSL4 expression levels via immunoprecipitation (IP) of proteins from each lysate sample with anti-ACSL4 antibody or control antibody (rabbit IgG) in the presence or absence of the proteasome inhibitor MG132 (20 μM). F IP of lysates with anti-ACSL4 antibody or control antibody (rabbit IgG) in the presence of the proteasome inhibitor MG132 (20 μM), and analysis of ubiquitinated ACSL4 in IP complexes using anti-ACSL4 and anti-ubiquitin antibodies. Data are presented as mean ± SD (n = 3 experiments); *p < 0.05, **p < 0.01, ***p < 0.001.

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