Fig. 2: TRIP13 is essential for cell proliferation in Gastric cancer.

A qRT-PCR assay and western blot assays were performed to detect TRIP13 expression in TRIP13-knocked-down MKN45 and HGC27 cells. B Growth experiment was used to detect the effect of knocking down TRIP13 on the proliferation ability of gastric cancer cells. C, D Morphology and numbers of cells were observed after knocking down TRIP13. E Cells positive for Edu staining were shown in knocking down TRIP13 MKN45 and HGC27 cells. F Cells positive for Edu staining after overexpression of TRIP13 in TRIP13-knockdown gastric cancer cells are shown. G Clone-forming ability was observed in knocking down TRIP13 gastric cancer cells. H Colony-formation was performed in TRIP13-overexpressing in TRIP13-knockdown gastric cancer cells. I The volcano plot analysis of transcriptomics revealed the number of upregulated and downregulated genes in MKN45 cells after knocking down TRIP13. J Western blot was performed to detect the protein expression of TRIP13, CDK2, and CCNE1 in TRIP13 knockdown cells. K Western blot was performed to detect the protein expression of TRIP13, CDK2, and CCNE1 in TRIP13-overexpressing in TRIP13-knockdown gastric cancer cells. All data were expressed as mean ± SD. The student’s t-test was performed to analyse the significance. *p < 0.05, **p < 0.01, ***p < 0.001.