Fig. 3: TRIP13 facilitates gastric cancer cell migration and invasion in vitro and metastasis in vivo.

A, B Wound-healing assay was performed with TRIP13-knockdown MKN45 and HGC27 cells. C, D Wound-healing experiment was conducted in gastric cancer cells that rescued TRIP13 expression after knocking down TRIP13. E, F Western blot experiments were performed to detect the protein expression of TRIP13, E-cadherin, N-cadherin and Snail in gastric cancer cells that were knocked down and rescued after knocking down TRIP13. G–N Transwell assay was performed to detect the migration and invasion of gastric cancer cells that were knocked down and rescued after knocking down TRIP13. O, P The tail vein injection assay in mice was adopt to detect the effects of knocking down TRIP13 and rescuing TRIP13 expression after knocking down TRIP13 on the in vivo metastasis ability of gastric cancer cells in mice. All data were expressed as mean ± SD. The student’s t-test was performed to analyse the significance. *p < 0.05, **p < 0.01, ***p < 0.001.