Fig. 1: Downregulated ETHE1 is observed in colorectal cancer and associated with a worse prognosis.

A The mRNA level of ETHE1 in CRC tissues compared with adjacent normal tissues using the TCGA-COAD and -READ datasets from the GEPIA online tool. Colon adenocarcinoma (COAD), and Rectum adenocarcinoma (READ). Student’s two-tailed test, p < 0.05. B–D The mRNA level of ETHE1 in CRC tissues compared with adjacent tissues using the GSE25070, GSE44861, and GSE20916 datasets. Student’s two-tailed t test, p < 0.001. E The protein level of ETHE1 detected by IB assay in 12-paired colorectal tumor and adjacent normal tissues (Left) and quantitative analysis of the 12-paired colorectal tumor and adjacent normal tissues (Right), paired t test, p = 0.002. F Representative images of ETHE1 expression from the CRC tissues microarray (TMA). Scale bar of left panels = 500 μm and scale bar of right panels = 100 μm. G Quantitative analysis of The IHC score of ETHE1 in the TMA. Student’s two-tailed t test, p < 0.001. H Kaplan–Meier Disease specific survival (DFS) analysis in the GSE17536 dataset according to ETHE1 expression. Log-rank test, p = 0.0343. I Kaplan–Meier Overall survival (OS) analysis in the TCGA-COAD and -READ datasets according to ETHE1 expression from the GEPIA online tool. Log-rank test, p = 0.028. J Kaplan–Meier Overall survival (OS) analysis according to ETHE1 expression in the TMA. Log-rank test, p = 0.0086. K Cox regression analysis shows the significance of the relationship between ETHE1 expression and prognosis for patients with CRC in the presence of other clinical variables.