Fig. 2: ETHE1 inhibits VEGF-A expression and curbs tumor angiogenesis in vitro. | Cell Death & Disease

Fig. 2: ETHE1 inhibits VEGF-A expression and curbs tumor angiogenesis in vitro.

From: ETHE1 dampens colorectal cancer angiogenesis by promoting TC45 Dephosphorylation of STAT3 to inhibit VEGF-A expression

Fig. 2

A GSEA enrichment plots demonstrate the correlation between the expression of ETHE1 and Angiogenesis (Up) or VEGF pathway (Down) in the TCGA COAD and READ datasets. B The protein level of VEGF-A detected by IB assay in control or ETHE1 stable overexpression SW48 and SW480 cells. C The protein level of VEGF-A detected by IB assay in SW48 and HCT116 cells silenced with control (shNC) or ETHE1 shRNA (#1 and #2). D The mRNA level of VEGF-A detected by qRT-PCR assays in control or ETHE1 stable overexpression SW48 and SW480 cells (n = 3). E The mRNA level of VEGF-A detected by qRT-PCR assays in SW48 and HCT116 cells silenced with control (shNC) or ETHE1 shRNA (#1 and #2) (n = 3). F Quantitative analysis of Transwell cell migration assay in HUVECs treated with the CM from control or ETHE1 stable overexpression SW48 and SW480 cells (n = 3). G The viability of HUVECs treated with the CM from control or ETHE1 stable overexpression SW48 and SW480 cells evaluated by using CCK8 assay (n = 3). H, J Representative images of tubule formation assay in HUVECs treated with indicated CM. Green scale bar, 100 μm. I, K Quantitative analysis of tubule formation from (H) and (I) (n = 3). All immunoblots were performed three times, independently, with similar results. Data are represented as mean ± s.d. *p < 0.05, **p < 0.01, ****p < 0.0001, by (D, F, I) Student’s two-tailed t test and (E, K) one-way ANOVA with Tukey’s test and (G) two-way ANOVA with Tukey’s test.

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