Fig. 3: ETHE1 restrains CRC tumor growth and tumor angiogenesis in vivo. | Cell Death & Disease

Fig. 3: ETHE1 restrains CRC tumor growth and tumor angiogenesis in vivo.

From: ETHE1 dampens colorectal cancer angiogenesis by promoting TC45 Dephosphorylation of STAT3 to inhibit VEGF-A expression

Fig. 3

A Representative image of subcutaneous tumors formed by control or ETHE1 stable overexpression SW480 cells in nude mouse xenograft models. B, C Quantification of tumor weight and tumor volume generated in (A) (n = 5). D Representative image of subcutaneous tumors formed by SW48 cells silenced with control (shNC) or ETHE1 shRNA (#1 and #2) in nude mouse xenograft models. E, F Quantification of tumor weight and tumor volume generated in (D) (n = 5). G IHC staining of xenograft tumors generated by control and ETHE1-overexpression SW480 cells using anti-ETHE1, anti-VEGF-A, and anti-CD31 antibodies. Black scale bar, 50 μm. H Quantitative analysis of the Microvessel density of subcutaneous tumors according to the staining status of CD31 (n = 5). I Representative image of the CM-Matrigel plugs formed after 7 days of subcutaneous injection in nude mice. Black scale bar, 1 cm. J Quantification of hemoglobin generated in (I) (n = 3). Data are represented as mean ± s.d. ***p < 0.001, ****p < 0.0001, by (C, H, J) Student’s two-tailed t test and (F) one-way ANOVA with Tukey’s test and (B, E) two-way ANOVA with Tukey’s test.

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