Fig. 6: ETHE1 hinders STAT3 activation by enhancing the interaction of TC45-STAT3. | Cell Death & Disease

Fig. 6: ETHE1 hinders STAT3 activation by enhancing the interaction of TC45-STAT3.

From: ETHE1 dampens colorectal cancer angiogenesis by promoting TC45 Dephosphorylation of STAT3 to inhibit VEGF-A expression

Fig. 6

A, B Indicated cells were treated with or without IL-6 (10 ng/mL) for 30 min, followed by various durations of starvation (indicated as withdrawal). WCL were collected for IB analysis. C HEK293T cells were transfected with Flag-ETHE1. WCL were collected for IP with anti-Flag beads, followed by IB analysis. D HEK293T cells were transfected with Myc-TC45 and Flag-ETHE1 plasmids, WCL were collected for IP with anti-Myc beads or anti-Flag beads, followed by IB analysis. E WCL of SW48 cells were collected for IP with anti-TC45 or anti-ETHE1 antibody, followed by IB analysis. F HEK293T cells were transfected with HA-STAT3 plasmid, then transfected with vector, Flag-ETHE1, or Myc-TC45 plasmids. WCL were collected for IP with anti-HA beads, followed by IB analysis. G, H WCL were collected from the SW48 and SW480 cells and conducted by IP assay using IgG or anti-STAT3 antibody, followed by IB analysis. I In vitro binding assay was performed. Purified Flag-STAT3 was incubated with TC45 in the absence or presence of purified His-ETHE1 and pulled down using anti-Flag beads, followed by IB analysis. J, K The protein level of VEGF-A, T-STAT3 and p-STAT3 detected by IB assay in the indicated cells treated with or without silencing TC45. All immunoblots were performed three times, independently, with similar results.

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