Fig. 1: Construction of the B lymphocyte lineage expression profile of MM patients. | Cell Death & Disease

Fig. 1: Construction of the B lymphocyte lineage expression profile of MM patients.

From: Single-cell sequencing analysis of multiple myeloma heterogeneity and identification of new theranostic targets

Fig. 1

A Workflow. Bone marrow samples from 7 patients with multiple myeloma were selected for scRNA-seq. CD34+/CD19+/CD138+ cells enriched by MACS were further sorted and detected using flow cytometry. Cells were analyzed using the 10× Genomics Chromium scRNA-seq platform. B Expression of four characteristic marker genes (CD34, MME, CD79B, and SDC1) during B-cell development. C Unified manifold approximation and projection (UMAP) representation of single-cell gene expression, showing the 12 identified cell types. Cells were color-coded based solely on cluster annotation. D Expression heatmap of characteristic marker molecules for each cell subset. E Correlation analysis map of each cell subset. F BP enrichment results of characteristic expressed genes for each cell subset.

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