Fig. 5: Biological effects of WNK1 on MM cells. | Cell Death & Disease

Fig. 5: Biological effects of WNK1 on MM cells.

From: Single-cell sequencing analysis of multiple myeloma heterogeneity and identification of new theranostic targets

Fig. 5

A Expression levels of WNK1 in the remaining bone marrow samples from normal donors and MM patients were determined using qRT-PCR. B The expression levels of WNK1 in the remaining bone marrow samples from normal donors and MM cell lines were detected using Western blot analysis. C Cell viability analysis of MM cell lines and bone marrow samples from MM patients. D Growth curves of MM cell lines treated with WNK-IN-11. E Colony formation experiments were conducted on MM cell lines treated with WNK-IN-11. F Statistics of clone numbers of MM cell lines. G Effect of WNK-IN-11 on the cell cycle distribution of MM cell lines, as determined by flow cytometry. H GLUT1 expression on the membrane surface was determined. GAPDH was used as the internal reference for plasma proteins, and ITGB1 was used as the internal reference for membrane proteins. Statistical analyses of n = 3 independent experiments were assessed. *p < 0.05, **p < 0.01, ***p < 0.001.

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