Fig. 5: Phosphorylation of the GSK3β Ser9 site by PIM1 suppresses SNAIL and SLUG degradation. | Cell Death & Disease

Fig. 5: Phosphorylation of the GSK3β Ser9 site by PIM1 suppresses SNAIL and SLUG degradation.

From: PIM1 kinase promotes EMT-associated osimertinib resistance via regulating GSK3β signaling pathway in EGFR-mutant non-small cell lung cancer

Fig. 5

A, B The influence of PIM1 knockdown and restoration on p-GSK3β (Ser9), p-GSK3β (Tyr216), GSK3β, GSK3α, SNAIL and SLUG was analyzed by immunoblotting. C, D Analysis of SNAIL and SLUG expression via immunoblotting in H1975/OR and PC9/OR cells transfected with GSK3β WT and GSK3β S9A plasmids. E, F The efficacy of PIM1 knockdown and the addition of siGSK3β or LiCl on the expression levels of SNAIL and SLUG in H1975/OR and PC9/OR cells was determined by immunoblotting. G, H IC50 assay of H1975/OR-shPIM1 cells and PC9/OR-shPIM1 transfected with siGSK3β. Data are presented as mean ± SD, **p < 0.01, ****p < 0.0001, One-way ANOVA. I, J Transwell assay of H1975/OR-shPIM1 cells and PC9/OR-shPIM1 transfected with siGSK3β. Data are presented as mean ± SD, **p < 0.01, ***p < 0.001, **** p < 0.0001, Two-way ANOVA.

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