Fig. 2: ALKBH5 increases in response to cardiomyocyte hypertrophy in vitro.
From: The functional role of m6A demethylase ALKBH5 in cardiomyocyte hypertrophy
A NRCMs were stimulated by phenylephrine (PE, 100 µM) to induce the hypertrophic responses in vitro and the protein levels of ALKBH5 and hypertrophic biomarkers ANP and BNP were examined by immunoblots. The quantifications were shown on the right. B The mRNA level of Alkbh5 gene in PE (100 µM, 24 h)-induced NRCMs was detected by qRT-PCR. C The protein levels of ALKBH5 and BNP were examined in PE-induced H9C2 cells by western blots. D The protein expressions of ALKBH5 and BNP were examined in ISO (30 µM)-induced H9C2 cells by western blots. n = 4 per group. Data are expressed as the mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
