Fig. 1: Gpx4 was identified by hamster cDNA expression screening as a gene rendering cold resistance to a human cancer cell line.
From: Identification of genes supporting cold resistance of mammalian cells: lessons from a hibernator
a Cold resistance of hamster HapT1 cells but not of human HT1080 cells. The proportion of dead cells was determined by LDH assay (one-way ANOVA with the Tukey’s multiple comparison test, p < 0.05). b Schematic illustration of hamster cDNA expression screening for genes that render the resistance to cold-rewarming culture to human HT1080 cells. c Venn diagram of the genes inserted in the genome of survived cells under cold-rewarming culture in four screening experiments. d The proportion of dead cells in HT1080 cells after 24 h of cold (4 °C) culture when each indicated gene was overexpressed (N = 3 wells, One-way ANOVA with the Dunnett’s multiple comparison test, ***p < 0.001).
