Fig. 2: NKp46⁺ILC3 orchestrates pathological phenotypic alterations in mouse colitis induced by in vivo ferroptosis interventions, independent of T cell involvement.

a Body weight changes of indicated groups of mice. (n = 3) b Statistical results of spleen weights at day 8 post infection. (n = 4) c The statistical results of histological scores are shown. (n = 4) d Colon lengths of indicated mice. (n = 4) Log₁₀ CFU of C.R in e liver and f spleen tissues. (n = 4) g The gating strategy for intestinal CD4⁺RORγt⁺ cells and ILC3s. h Statistical results of the proportion (upper) and absolute number (lower) of CD4⁺RORγt⁺ cells in LPMCs from the indicated groups of mice. (n = 4) i The representative flow cytometry plots and statistical results of the proportion and absolute number of j ILC3s, including k NKP46⁺ILC3, l DN cell subsets, and m CCR6⁺ILC3 in LPMCs of indicated mice. (n = 4) n Representative FACS plots of IL-22- (upper) and IL-17A-positive (lower) ILC3s in LPMCs from the indicated groups of mice. Statistical results of proportion (left) and absolute numbers (right) of o IL-22-, and p IL-17A-expressing ILC3s are shown. (n = 4) q Statistical results of the proportion (left) and numbers (right) of neutrophils in LPMCs from the indicated groups of mice. (n = 4) r Relative RegIIIβ and RegIIIγ mRNA expression in the colon tissue of indicated mice. (n = 3) s The percentage of annexin V and 7-AAD double-negative population in LPMCs-derived ILC3s from the indicated groups of mice. (n = 4) t Statistical results of lipid ROS production in ILC3s are shown. (n = 4) u The statistical results of MFI for GPX4 in ILC3s from LPMCs of indicated mice. (n = 4) Data are presented as the mean ± SEM or median, and statistical significance was determined by one-way ANOVA test (b–f, h, j–m, o–u). *P < 0.05; **P < 0.01; ***P < 0.001.