Fig. 7: IDO1 inhibitor (IDO1i) could reverse the tumor‑promoting effects of MSCs in vitro and in vivo. | Cell Death & Disease

Fig. 7: IDO1 inhibitor (IDO1i) could reverse the tumor‑promoting effects of MSCs in vitro and in vivo.

From: Mesenchymal stem cells in tumor microenvironment: drivers of bladder cancer progression through mitochondrial dynamics and energy production

Fig. 7

Immunoblot showed that the expression of IDO1 decreased significantly in MSCs with the increased concentration of linrodostat (a). Colony formation assays (b) and wound-healing assays (c) confirmed that IDO1i can impede the pro-tumorigenic effects of MSCs in UMUC-3 cells. The growth rate of tumor volume was slow in IDO1i and MSC+IDO1i groups (d), and tumor weight also showed a significant decrease in IDO1i and MSC+IDO1i groups (e). No significant difference in body weight was observed in four groups (f). In the presence of linrodostat, MSCs failed to induce MMP hyperpolarization in UMUC-3 cells (g). TEM showed that MSCs improved mitochondrial morphology and increased mitochondrial numbers, which could be disrupted by linrodostat (h). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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