Fig. 4: Gαi3 KO causes robust anti-pancreatic cancer cell activity.
From: Identification of Gαi3 as a promising molecular oncotarget of pancreatic cancer
Primary priPC-1 cells and immortalized BxPC-3 cells were subjected to transduction with two distinct lentiviral CRISPR/Cas9 constructs targeting Gαi3 for KO (“ko-Gαi3 “, with s1/s2 representing two distinct sgRNAs), in comparison to a control group transduced with an empty CRISPR/Cas9 vector (“Cas9-C”). Subsequently, stable cells were generated, and the listed proteins were evaluated (A–G). Cells were then cultured for additional specified time periods, cell viability (B–H), proliferation (C–I), cell cycle progression (D), migration (E–J), and invasion (F) were tested, with results quantified. *P < 0.05 versus “Cas9-C” group. “N. S.” stands for P > 0.05. The experiments depicted in this figure were replicated five times (n = 5, biological repeats), consistently yielding similar results. Scale bar = 100 μm.
