Fig. 1: NOD2 is lowly expressed in melanoma and suggests a poor prognosis for melanoma patients.
From: NOD2 reduces the chemoresistance of melanoma by inhibiting the TYMS/PLK1 signaling axis
A GEPIA database analysis of NOD2 mRNA expression levels in normal and melanoma tissues. B TCGA database analysis of NOD2 mRNA expression levels in normal, primary, and metastatic melanoma tissues. C Correlation between NOD2 expression in the UALCAN database and prognosis of melanoma patients. D, E mRNA of NOD2 in human melanocytes HEM and melanoma cells A375, A875, SK-MEL-28, and SK-MEL-110 was detected by real-time PCR (D), and the protein expression level was detected by Western blot (E). F–H Construction of stably transfected cells with NOD2 knockdown and overexpression in A875 and SK-MEL-110. Protein expression levels were detected by Western blot (F, G), and mRNA expression levels were detected by real-time PCR (H). Data are expressed as the mean ± SD. Student’s t-test and one-way ANOVA were used to compare the differences. *P < 0.05, **P < 0.01, ***P < 0.001. ns not significant.
