Fig. 5: NOD2 inhibits human melanoma growth in vivo.
From: NOD2 reduces the chemoresistance of melanoma by inhibiting the TYMS/PLK1 signaling axis
A–C 1 × 107 of A875 cells with NOD2 overexpression and control (OE-NC) were injected into the left (OE-NC) and right (OE-NOD2) sides of BALB/c nude mice (n = 4 per group). After tumor formation, mice were euthanized, and photographs of the tumors were taken (A). Tumor volume was monitored, and growth curves were plotted (B). Tumor weight was measured (C). D–F 1 × 107 of A875 cells with NOD2 knockdown and control (sh-NC) were injected into the left (sh-NC) and right (sh-NOD2) sides of BALB/c nude mice (n = 4 per group). After tumor formation, mice were euthanized, and photographs of the tumors were taken (D). Tumor volume was monitored, and growth curves were plotted (E). Tumor weight was measured (F). G NOD2 expression and related cyclins were detected by Western blot. H HE staining and IHC staining of the tumor. Scale bar = 50 μm. Data are expressed as the mean ± SD. Student’s t-test and one-way ANOVA were used to compare the differences. *P < 0.05, **P < 0.01, ***P < 0.001.
