Fig. 7: NOD2 negatively regulates TYMS protein abundance by promoting the K48-linked ubiquitination of TYMS.
From: NOD2 reduces the chemoresistance of melanoma by inhibiting the TYMS/PLK1 signaling axis
A–C Western blot detection of protein levels of TYMS in stably transfected A875 and SK-MEL-110 cells treated with cycloheximide (CHX) (100 μg/mL) with or without MG-132 (10 μg/mL) and CQ (20 μM) for 0, 3, 6, and 9 h. D CO-IP assay detected the ubiquitination expression level of TYMS in stably transfected A875 and SK-MEL-110 cells. E, F CO-IP assay detected the K48-linked (E) and K63-linked (F) ubiquitination expression levels of TYMS in stably transfected A875 and SK-MEL-110 cells. Data are expressed as the mean ± SD. Student’s t-test and one-way ANOVA were used to compare the differences, ***P < 0.001.
