Fig. 2: PVT1 deletion ameliorated podocyte injury in STZ-induced diabetic mice.

A Temporal changes in body weights were measured respectively at 4 weeks (n = 6), 6 weeks (n = 6), 8 weeks (n = 5), 12 weeks (n = 5), and 20 weeks (n = 5) after DKD initiation. *P < 0.05 vs CTL-Cre⁺/Pvt1^+/+, ^#P < 0.05 vs STZ-Cre⁺/Pvt1^+/+. B Kidney weight to body weight ratio was elevated in mice at 20 weeks after DKD initiation (n = 5). C Temporal urinary albumin-to-creatinine ratio (ACR) of mice were detected respectively at 6 weeks (n = 6), 8 weeks (n = 5), 12 weeks (n = 5), 16 weeks (n = 5), and 20 weeks (n = 5). *P < 0.05 vs CTL-Cre⁺/Pvt1^+/+, ^#P < 0.05 vs STZ-Cre⁺/Pvt1^+/+. D Representative fluorescence images of WT-1 in glomeruli isolated from the four groups at 20 weeks after DKD initiation (n = 5). Scale bar = 50 μm. E PAS staining of glomeruli from the four groups at 20 weeks after DKD initiation (n = 5). Scale bar = 50 μm. F TEM image of podocytes and, GBM, and mitochondria in primary podocytes from the four groups at 20 weeks after DKD initiation (n = 5). G Expression of TFAM, BAX, DRP1, and mitochondrial OXPHOS proteins in primary podocytes from the four groups at 20 weeks after DKD initiation (n = 3). H Measurement of the mitochondrial OCR of primary podocytes from mice at 20 weeks after DKD initiation (n = 5). I Relative mRNA levels of Il-6, Tnf-α, and Cxcl10 in mice at 20 weeks after DKD initiation (n = 5). Error bars represent the mean ± S.D, *P < 0.05, **P < 0.01, and ***P < 0.001.