Fig. 4: DNA methylation on the p16 CGI promoter is reversely correlated with PRC2 occupancy.

A Methylation-specific PCR analysis on the p16 CGI promoter region. M means PCR product for methylated CGI region, U means PCR product for unmethylated CGI region. B ChIP-qPCR analysis on the occupancy of H3K27me3 and EZH2 at the p16 CGI region of DMS 114, MFE-296, NCI-H23, and TOV-112D cells with or without BR0063 incubation. C The expression level of TET1 in the tested SWI/SNF LOF tumor cells analyzed by qPCR, each sample was run in triplicate, and the error bars represent the mean ± SD.