Fig. 2: Nme8−/− male mice exhibited normal fertility. | Cell Death & Disease

Fig. 2: Nme8−/− male mice exhibited normal fertility.

From: Nme8 is essential for protection against chemotherapy drug cisplatin-induced male reproductive toxicity in mice

Fig. 2

A Construction diagram of Nme8−/− mice. B PCR was performed to determine mice genotypes. C The sequencing results of the PCR product from Nme8−/−mice. D, E Immunohistochemistry analysis and western blot confirmed successful knockout of the NME8 protein. Scale bar = 50 μm. F Schematic illustration of two transcripts of Nme8. G, H Agarose gel electrophoresis results of the PCR products. I Body weights of adult WT and Nme8−/− mice. n = 4 biologically independent mice. J The number of offspring from WT or Nme8−/− male mice by cohabiting with WT female mice. n = 3 biologically independent mice. K, L The morphology and weights of the testis and epididymis were assessed in adult WT and Nme8−/− mice. Scale bar = 1 mm. I, J, and L NS indicates non-significant. M, N H&E staining was used to examine the testis, epididymis, and sperms of adult mice. Scale bar = 50 μm. O, P Sperm deformity and motility were evaluated in WT and Nme8−/− mice. NS indicates non-significant, n = 3 biologically independent mice.

Back to article page