Fig. 5: Nme8 deficiency affects spermatogonia differentiation and spermatocyte meiosis under CP stimulation. | Cell Death & Disease

Fig. 5: Nme8 deficiency affects spermatogonia differentiation and spermatocyte meiosis under CP stimulation.

From: Nme8 is essential for protection against chemotherapy drug cisplatin-induced male reproductive toxicity in mice

Fig. 5

A, B The immunofluorescence staining and statistical results of DDX4. Scale bar = 50 μm. C, D The immunofluorescence staining and statistical results for PCNA in mice testis. Scale bar = 50 μm. E, F Immunohistochemistry of c-KIT was used to test differentiating spermatogonia. Scale bar = 20 μm. G γH2AX was used to test the spermatocyte meiosis in the testis. Scale bar = 100 μm. The yellow and white asterisks marked the tubules that abundantly and punctately express γH2AX, respectively. H The proportion of tubes with extensive γH2AX expression in all tubes. I, J The number of cells with extensive (I) and punctate γH2AX (J) in each tubule. K The protein expression of γH2AX in the testis of different groups of mice. L, M SOX9 was used to mark Sertoli cells in testis. *P < 0 .05, **P < 0.01, ***P < 0.001, NS indicates non-significant, n = 4 biologically independent mice in each group.

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