Fig. 7: The deletion of Nme8 results in elevated DNA damage in the testes and sperm after CP stimulation. | Cell Death & Disease

Fig. 7: The deletion of Nme8 results in elevated DNA damage in the testes and sperm after CP stimulation.

From: Nme8 is essential for protection against chemotherapy drug cisplatin-induced male reproductive toxicity in mice

Fig. 7

A The relative levels of 8-OHdG in the testes of WT, WT + CP, and Nme8−/−+CP mice were measured. n = 3 biologically independent mice in each group. B, C The TUNEL assay was used to test the DNA damage in the testis. Scale bar = 20 μm, n = 4 biologically independent mice in each group. D The enlarged view inside the white dotted box in (B). Scale bar = 20 μm. E, F SCD was used to analyze DNA damage in sperms from WT, WT + CP, and Nme8−/−+CP mice, and the degree of DNA fragmentation was assessed by comparing halo and nuclear sizes. Scale bar = 10 μm. ##P < 0.01, ###P < 0.001 vs WT; *P < 0 .05, **P < 0.01, ***P < 0.001 vs WT + CP; n = 4 biologically independent mice in each group. G–J The autophagy related proteins LC3, ATG5, and P62 were tested in the testis. *P < 0 .05, **P < 0.01, ***P < 0.001, n = 4 biologically independent mice in each group.

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