Fig. 1: SIRT-1 expression is compromised in dendritic cells exposed to a high-fat diet.
From: Sirtuin 1 regulates the phenotype and functions of dendritic cells through Ido1 pathway in obesity
Bone marrow-derived dendritic cells (BM-DCs) were generated from mice under standard diet (SD) and high-fat diet (HFD). qPCR of Sirt1 from BMDCs from SD and HFD (A). Confocal microscopy of BMDCs/SD and BMDCs/HFD, showing SIRT1 (red) on BMDCs/SD and HFD (B). Flow cytometry representing the SIRT1 MFI (mean fluorescence intensity MFI) and percentage of SIRT1+ BMDCs (gated on Live & Dead-CD11c+IA/IE+CD115+) from SD and HFD animals, with or without RES (50 µM/24 h) and EX-527 (10 µM/24 h) (C–E). SIRT1 activity fluorometric assay of total protein extract from BMDCs from SD and HFD mice, formerly treated with RES (50 µM/24 h) or EX-527 (10 µM/24 h) or untreated (F). Significance values (p) are indicated as follows: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001, One-way ANOVA or t-test when necessary, using GraphPad Prism®. The graphs and illustrations represent a representative experiment of three distinct experiments with 3–6 animals per group.
