Fig. 2: Pro-inflammatory activation of dendritic cells by a high-fat diet is inversely related to SIRT1 expression.
From: Sirtuin 1 regulates the phenotype and functions of dendritic cells through Ido1 pathway in obesity
Bone-marrow-derived Dendritic cells (BM-DCs) were generated from mice on a standard diet (SD) and a high-fat diet (HFD). MHC-IIhigh (top gate) and MHC-IIlow (bottom gate) contour plot from flow cytometry of BMDCs from SD and BMDCs from HFD (with RES [50 µM], EX-527 [10 µM], or no treatment) (A). Quantification of percentage (B) and MFI (C) (mean fluorescent intensity) of MHC-IIhigh is represented. MFI (mean fluorescent intensity) of CD86 (D) and CD40 (E) from the same BMDCs under the mentioned conditions. Heat-map representing qPCR data (2-ΔΔCT) of the main cytokines produced by BMDCs/SD and BMDCs/HFD, untreated or treated with RES [50 µM] and EX-527 [20 µM] for 24 h (F). Heat-map of cytokine production was measured by ELISA for IL-1β, IL-6, IL-12p40, IL-10, and TGF-β in BMDCs from SD and HFD under the same conditions (G). The significance values (p) are indicated as follows: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001, determined by One-way ANOVA or t-test when necessary, using GraphPad Prism®. The graphs and illustrations represent a representative experiment of three distinct experiments, with 3–6 animals per group.
