Fig. 3: High-fat diet-induced pro-inflammatory DC favors Th1 generation by SIRT1 levels.
From: Sirtuin 1 regulates the phenotype and functions of dendritic cells through Ido1 pathway in obesity
Experimental design representation for BMDCs (OVA pulsed)-CD4 T cells (OT-II) co-culture approach (A). Histogram from flow cytometry representing Cell-Trace Violet-labeled OT-II CD4 T cells proliferation, which were co-cultured (72 h) with OVA-pulsed BMDCs/SD and BMDCs/HFD under RES [50 µM], EX-527 [10 µM] treatment, or untreated (CT) (B). Percentage of OT-II CD4 T cells proliferation (Cell-Trace Violet) after co-culture (72 h) with BMDCs/SD and BMDCs/HFD under treatment or no treatment (RES, EX-527, or CT respectively) (C). Pseudo color plot (D), percentage (E), and MFI mean fluorescence intensity) (F) of IFN-γ production from CD4 T cells (OT-II) co-cultured with BMDCs/SD and BMDCs/HFD under RES, EX-527 treatment, or no treatment (CT). Pseudo color smooth plot (G), percentage (H) of Foxp3 and CD25, and MFI (MFI mean fluorescence intensity) (I) of regulatory CD4 T cells (OT-II) previously co-cultured with BMDCs/SD and BMDCs/HFD under RES, EX-527 treatment, or no treatment. Percentage of CD44 (J) and MFI (mean fluorescence intensity) of CD44 (K) from CD4 T cells (OT-II) co-cultured with BMDCs/SD and BMDCs/HFD treated (RES or EX-527) or untreated. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001, determined by One-way ANOVA or t-test when necessary, using GraphPad Prism®. The graphs and illustrations represent a representative experiment of three distinct experiments, with 3–6 animals per group.
