Fig. 8: Conditional knockout of Sirt1 in dendritic cells disrupts global metabolism, possibly affecting NAD+ production via the kynurenine pathway. | Cell Death & Disease

Fig. 8: Conditional knockout of Sirt1 in dendritic cells disrupts global metabolism, possibly affecting NAD+ production via the kynurenine pathway.

From: Sirtuin 1 regulates the phenotype and functions of dendritic cells through Ido1 pathway in obesity

Fig. 8

Body weight (g) from SIRT1Δ and control mice (SIRT1 flox) on a standard diet (SD) and high-fat diet (HFD) (A). Blood glucose tolerance test (GTT) levels, including Area Under the Curve (AUC) data (B, C). Insulin resistance test with AUC (D, E). Mean Fluorescent Intensity (MFI) of MHC-II, CD40, and ELISA of IL-12p40 in BMDCs (with or without 2 mM of Acetylated-NAD) extracted from SIRT1Δ and SIRT1 flox mice after 12 weeks on SD or HFD (F–H). Ido1 qPCR in BMDCs from SIRT1Δ and SIRT1 flox mice on SD and HFD (with or without 2 mM of Acetylated-NAD) (I). Intracellular flow cytometry staining of Peroxisome Proliferator-Activated Receptor Gamma (PPARγ) for percentage (J) and MFI (K) in BMDCs from SIRT1Δ and SIRT1 flox mice on SD and HFD. Significance values are indicated as follows: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Statistical analyses were performed using ANOVA or t-test where appropriate, with data analyzed using GraphPad Prism®. The graphs and illustrations represent data from one to three independent experiments, with 2–6 animals per group.

Back to article page