Fig. 3: Effect of exercise on the Wnt/Ca2+ signaling pathway and synaptic transmission in HFD-fed mice. | Cell Death & Disease

Fig. 3: Effect of exercise on the Wnt/Ca2+ signaling pathway and synaptic transmission in HFD-fed mice.

From: Regular exercise ameliorates high-fat diet-induced depressive-like behaviors by activating hippocampal neuronal autophagy and enhancing synaptic plasticity

Fig. 3

A Representative immunofluorescence image of neurons in the hippocampal CA1 region labeled with Wnt5a and NeuN (bar = 20 μm). B Statistical analysis of the ratio of Wnt5a to NeuN colocalization (n = 4). C Representative images of the western blot and D relative Wnt5a protein expression levels and E protein expression levels of p-CamkII-α/CamkII-α in the different groups (n = 3). Gapdh served as control. Full and uncropped western blots are detailed in the supplementary material. F Electrophysiological schematic. G Sample traces of excitatory postsynaptic current (EPSC). H Cumulative probability and comparison of the means of the EPSC amplitude (n = 8 from 4 mice in each group). I Cumulative probability and mean EPSC frequency (n = 8 from 4 mice in each group). All the results are presented as the means ± standard deviations (SDs) with statistical significance (*P < 0.05, **P < 0.01, ns: P > 0.05).

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