Fig. 1: miR-200c-3p downregulation is associated with radioresistance.

A Radiosensitivity of established radiation-resistant and parental cells was evaluated by colony formation assay. RPF: Radiation Protection Factor. Means and SEM are represented. Wilcoxon test, one side: *p < 0.05, **p < 0.01. n = 6 independent experiments. B Volcano plot showing the pairwise comparisons of the differential expression of miRNAs in DU145 and PC3 sensitive and RR cells. The red dots on the left represent miRNAs that were significantly downregulated in the radioresistant cells compared to the parental cells. In green, miRNAs with a log2FC > 1 or <−1 are indicated. In red, miRNAs with an adjusted p value < 0.05. C Log2 Fold Change of miRNAs expressions in DU145 and PC3 cells compared to DU145 RR and PC3 RR cells measured by RT-qPCR (n > 4). D Characterization of the small EVs released by prostate cell lines: Western blot analysis (3 µg of sample was loaded per well. CD63, CD81, and TSG101 proteins are exosomes markers, and calnexin is a cytosolic protein), scanning electron microscopy (SEM) and TRPS technology. In the electron microscopy image, the arrows indicate intact vesicles, while the arrowhead shows amorphous material compatible with protein aggregates. In the TRPS plot, each replicate is represented by a gray level. E Volcano plot of differentially expressed miRNAs in small EVs from DU145 and DU145 RR cells. The red dots on the left represent significantly downregulated miRNAs in small EVs from the DU145 RR cells, and on the right, the upregulated miRNAs compared to small EVs from the DU145 cells. In green, miRNAs with a log2FC > 1 or <−1 are indicated. In red, miRNAs with an adjusted p value < 0.05. F Log2 Fold Change of miRNAs expressions in DU145 RR-derived small EVs compared to DU145-derived small EVs measured by RT-qPCR (n > 4).