Fig. 5: miR-200c-3p directly targets CBX5 mRNA. | Cell Death & Disease

Fig. 5: miR-200c-3p directly targets CBX5 mRNA.

From: Loss of miR-200c-3p promotes resistance to radiation therapy via the DNA repair pathway in prostate cancer

Fig. 5

A Relative expression of luminescence of HEK-293 cells transfected with 66 nM of miR-200c-3p (grey) or control miRNA (miR-neg) (white) and WT or mutated constructs in the miR-200c-3p binding site (20 ng). Luminescence expression is relative to the luminescence of miR-neg transfected cells. n = [4–5], Wilcoxon test one side, *p < 0.05. A schematic representation of the wild-type and mutated constructs in the miR-200c-3p-binding site is shown. WT, wild type. B Quantification of HP1α expression in DU145 RR cells transfected with miR-200c-3p or control miRNA (miR-neg) by western blot analysis. The optical density of each sample was measured and normalized using a nucleus housekeeping protein (H3) run on the same gel using ImageJ. The data are expressed as relative expression (HP1α/H3). n = 3, Wilcoxon test, *p < 0.05.

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