Fig. 6: Silencing of miR-200c-3p is associated with radiation resistance in other cancers.

A miR-200c-3p expression in lung cancer cell lines. The expression of miR-200c-3p is expressed relative to snord44, an endogenous housekeeping RNA. Means and SEM of n = 4 experiments per cell line are represented. B Relationships between radioresistance at 6 Gy and miR-200c-3p expression in lung cancer cell lines. Lung cancer cell lines were grouped according to their miR-200c-3p expression. Means and SEM of n = 3 experiments per cell line are shown. Wilcoxon test. C miR-200c-3p expression in breast cancer cell lines. The expression of miR-200C-3p is expressed relative to snord44, an endogenous housekeeping RNA. Means and SEM of n = 4 experiments per cell line are represented. D DNA methylation levels of the MIR200/141 promoter in lung and breast cancer cell lines measured by pyrosequencing. CpG sites are represented according to the beginning of the pre-miR-200c sequence. E Survival fraction at 6 Gy of A549 and ADCA72 cell lines transfected with miR-200c-3p or a control miRNA. 9 experiments were performed for each cell line. Wilcoxon test. F RT-qPCR of CBX5 mRNA in A549 and ADCA72 cell lines transfected with miR-200c-3p compared to a control miRNA. n = 7 experiments per cell line. *p < 0.05, **p < 0.01, ***p < 0.001.