Fig. 4: ROS serves as a pivotal factor in pyroptosis activation through NF-κB pathway in the context of NR5A2 deficiency. | Cell Death & Disease

Fig. 4: ROS serves as a pivotal factor in pyroptosis activation through NF-κB pathway in the context of NR5A2 deficiency.

From: Hepatocyte-specific NR5A2 deficiency induces pyroptosis and exacerbates non-alcoholic steatohepatitis by downregulating ALDH1B1 expression

Fig. 4

A, B Western blotting analysis was performed to detect the expression of pyroptosis-related molecules in the LO2-NR5A21in9397 (A) and AML12- Nr5a26in327 (B) cell lines after treated with gradient concentrations of inhibitors BAY11-7082 and MCC950. C, D GSEA was conducted to analyze the NF-κB pathway (C) and inflammation (D) using RNA-seq data from 5-week-old Nr5a2HKO mice model. E KEGG Enrichment Scatterplot of 1285 different expressed genes (DEGs), based on the RNA-seq data from 5-week-old Nr5a2HKO mice model. F MDA content assay was performed to measure liver and serum levels in the 5-week-old Nr5a2HKO mice model. N = 3/group, ns, not significant, *P < 0.05. Data are presented as means ± SD. G Serum MDA content assay of 8-week-old Nr5a2HKO mice model (3 technical replicates). N = 3/group, *P < 0.05. Data are presented as means ± SD. H ROS assay was carried out in the LO2-NR5A21in9397-C4 cell line and its negative control. N = 7/group, ****P < 0.0001. Data are presented as means ± SD.

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