Fig. 5: ALDH1B1 exhibited downregulation in the liver of Nr5a2HKO mice and was found to modulate the NF-κB signaling pathway. | Cell Death & Disease

Fig. 5: ALDH1B1 exhibited downregulation in the liver of Nr5a2HKO mice and was found to modulate the NF-κB signaling pathway.

From: Hepatocyte-specific NR5A2 deficiency induces pyroptosis and exacerbates non-alcoholic steatohepatitis by downregulating ALDH1B1 expression

Fig. 5

A Volcano plot. The plot displayed -log10(P value) versus log2(fold change) of 1285 differentially expressed genes (DEGs), with 1055 genes upregulated and 230 genes downregulated in the liver of 5-week-old Nr5a2HKO mice compared to their littermates. B ROS assay of LO2 cell line overexpressing ALDH1B1. CHO: cholesterol. Cholesterol was used at a concentration of 80 μg/mL for 24 h. pCDH refers to pCDH_EF1a-IRES-puro_BsrG1 vector. N = 3/group, *P < 0.05. Data are presented as means ± SD. C–F Western blotting (C), its statistical analysis (D), RT-qPCR assay (E), and immunohistochemical staining (F) were performed to evaluate Aldh1b1 expression in the liver of 12-week-old Nr5a2HKO mice model. N = 6/group, **P < 0.01, ****P < 0.0001. Data are presented as means ± SD. G, H Immunofluorescence staining was conducted to examine ALDH1B1 and phosphorylated NF-κB p65 levels in the AML12 (G) and LO2 (H) cell lines overexpressing ALDH1B1. Cholesterol was used at a concentration of 80 μg/mL for 24 h in the LO2 cell line, while it was used at a concentration of 320 μg/mL for 24 h in the AML12 cell line.

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