Fig. 7: ALDH1B1 can ameliorate the activation of pyroptosis, and NR5A2 different isoforms exhibit a similar function. | Cell Death & Disease

Fig. 7: ALDH1B1 can ameliorate the activation of pyroptosis, and NR5A2 different isoforms exhibit a similar function.

From: Hepatocyte-specific NR5A2 deficiency induces pyroptosis and exacerbates non-alcoholic steatohepatitis by downregulating ALDH1B1 expression

Fig. 7

A, B Western blotting analysis was performed to detect the expression of ALDH1B1 in the livers of NASH patients and control group (A), followed by statistical analysis (B). N = 6/group, data are presented as means ± SD. C The mRNA level of ALDH1B1 was measured in the livers of NASH patients and control group. N = 6/group, data are presented as means ± SD. D Immunohistochemistry staining was conducted to examine the expression of Aldh1b1 in the liver paraffin sections of BKS-db mice and BKS-NC mice. E, F The protein expression of pyroptosis molecules were assessed after overexpressing ALDH1B1 in the NR5A21in9397-C4 LO2 cell line (E) and AML12-Nr5a26in327 cell line (F). G, H The western blotting analysis was performed to evaluate the expression levels of ALDH1B1 and pyroptosis molecules after replenishment with different isoforms of NR5A2 in the LO2-NR5A21in9397-C4 cell line (G) and AML12-Nr5a26in327 cell line (H). I, J RT-qPCR was conducted to measure TGF-β mRNA expression in the LO2 cell line (I) and AML12 cell line (J), following haploid knockout or replenishment of NR5A2 with different isoforms. LO2: N = 4/group, AML12: N = 3/group, ns not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data are presented as means ± SD.

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