Fig. 6: VCP faciliate OS autophagy via USP2-mediated deubiquitination of FASN. | Cell Death & Disease

Fig. 6: VCP faciliate OS autophagy via USP2-mediated deubiquitination of FASN.

From: VCP enhances autophagy-related osteosarcoma progression by recruiting USP2 to inhibit ubiquitination and degradation of FASN

Fig. 6

A Western blot was conducted to analyze the levels of autophagy-related proteins, FASN, and FASN ubiquitination in 143B and U2OS cells stably transfected with Ca-NC, Ca-VCP or co-transfected with Ci-FASN or Ci-USP2. B Representative images and quantification revealing the immunofluorescence staining intensity with RFP-GFP-LC3 in 143B cells stably transfected with Ca-NC, Ca-VCP or co-transfected with Ci-FASN or Ci-USP2. Red (RFP + GFP-) puncta represent autolysosomes, and yellow (RFP + GFP + ) puncta represent autophagosomes. Scale bar: 20 µm. C Representative images from TEM scanning (left panel) and quantification (right panel) exhibiting autophagic vacuoles in in 143B cells stably transfected with Ca-NC, Ca-VCP or co-transfected with Ci-FASN or Ci-USP2 Scale bar: 20 µm. *P < 0.05 vs. Ca-NC + Ctrl.

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