Fig. 7: Immunoprecipitation of CHRNA5-CES1. | Cell Death & Disease

Fig. 7: Immunoprecipitation of CHRNA5-CES1.

From: Nicotine-induced CHRNA5 activation modulates CES1 expression, impacting head and neck squamous cell carcinoma recurrence and metastasis via MEK/ERK pathway

Fig. 7

A mRNA level validation after overexpression of CHRNA5. B Protein level validation after overexpression of CHRNA5. C Quantitative analysis of protein levels after overexpression of CHRNA5. D Immunoprecipitation validation of CHRNA5 and CES1 in Cal27 cells. E Immunoprecipitation validation of CHRNA5 and CES1 in Tu686 cells. F Confocal microscopy of triple-stained CHRNA5 (green), CES1 (red), and DAPI (blue) in Cal27 cells; G Scatter plot depicting the colocalization of CHRNA5 and CES1; H Line graph illustrating the colocalization of CHRNA5 and CES1.

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