Fig. 4: Effect of Ferrostatin-1 on renal damage in MRL/lpr mice with lupus nephritis. | Cell Death & Disease

Fig. 4: Effect of Ferrostatin-1 on renal damage in MRL/lpr mice with lupus nephritis.

From: OTUB1 regulation of ferroptosis and the protective role of ferrostatin-1 in lupus nephritis

Fig. 4

A Illustration of the experimental timeline for administering Ferrostatin-1 to 20-week-old MRL/lpr mice with established renal injury. B Measurement of urinary protein/creatinine ratio and serum creatinine levels post treatment, showing significant reductions, while total IgG and dsDNA levels in blood remained unchanged. C Hematoxylin and eosin (HE), Masson’s trichrome, Periodic acid-Schiff (PAS), and PASM (PAS with Methyl Green) staining. Scale bar: 20 μm. D Western blot and immunofluorescence analysis demonstrated increased expression of podocyte markers following Ferrostatin-1 treatment, indicating reduced podocyte injury. Scale bar: 20 μm. Data are represented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.

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