Fig. 2: Knockdown of USP11 promoted mitochondrial apoptosis. | Cell Death & Disease

Fig. 2: Knockdown of USP11 promoted mitochondrial apoptosis.

From: The USP11/Nrf2 positive feedback loop promotes colorectal cancer progression by inhibiting mitochondrial apoptosis

Fig. 2

A, B The relative mRNA expression levels of apoptosis-related molecules in the USP11 high- and low- expression group in the AOM/DSS mouse model and HCT116/DLD1 cells. HCT116/DLD1 cells were transfected with siRNAs to knock down USP1l. C HCT116/DLD1 cells were stained with Annexin V-FITC/ propidium iodide to examine cell apoptosis via flow cytometry. (n = 3). D Representative fluorescent images of JC-1 monomers and JC-1 aggregates in HCT116/DLD1 cells. (n = 3). JC-1 aggregates were stained red, which revealed intact mitochondria. JC-1 monomers, which show the reduction of the ΔΨm, were stained green. Scale bar: 50 μm. E Statistical analyses of cellular ATP generation. (n = 3). F The detection of mitochondrial fission protein. (n = 3). G Molecules associated with mitochondrial apoptosis: Cleaved PARP1, Procaspase 9, Cleaved Caspase 9, Bcl-2, Bak, Bax, Cleaved Caspase 3, and Cytochrome C levels were measured by western blotting. (n = 3).

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