Fig. 4: USP11 protected Nrf2 from degradation via deubiquitination. | Cell Death & Disease

Fig. 4: USP11 protected Nrf2 from degradation via deubiquitination.

From: The USP11/Nrf2 positive feedback loop promotes colorectal cancer progression by inhibiting mitochondrial apoptosis

Fig. 4

The endogenous interaction of USP11 and Nrf2 was tested in HCT116/DLD1 cell. Flag-USP11, His-Nrf2 plasmids and empty vectors were transfected alone or cotransfected into HEK293 T cells. A, B USP11/Nrf2 interaction was confirmed by endogenous and exogenous Co-IP assay. (n = 3). C The decline in Nrf2 protein levels could be reversed by MG132 (20 μM). (n = 3). D The half-life of Nrf2 was affected by USP11. HCT116/DLD1 cells were treated with CHX (50 μg/ml) and harvested at the indicated times. (n = 3). E Knockdown of USP11 led to an increase in the polyubiquitination level of Nrf2. (n = 3). Ubiquitin, Ubiquitin 48, His-Nrf2 plasmids and Flag-USP11 or empty vector were transfected into 293 T cells and Nrf2 ubiquitination was performed. F Functions of USP11 on Nrf2 ubiquitination in HEK293 T cells. USP11 decreased the K48-linked polyubiquitylation of Nrf2. (n = 3).

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