Fig. 6: USP11 knockdown suppressed CRC proliferation in vivo xenograft model. | Cell Death & Disease

Fig. 6: USP11 knockdown suppressed CRC proliferation in vivo xenograft model.

From: The USP11/Nrf2 positive feedback loop promotes colorectal cancer progression by inhibiting mitochondrial apoptosis

Fig. 6

A TBHQ, a widely used Nrf2 activator, could promote the expression of Nrf2 in HCT116 cell. Nude mice were randomized into three groups and subcutaneously injected with HCT116 cells that had been transfected with control, shUSP11, or shUSP11+TBHQ. B Representative photographs of tumors in nude mice were performed. C Body weight curves were measured for mice in the three groups. D, E Statistical analysis graph of the tumor volume and weight. F The expression of relative ROS levels, TBHQ could reverse the elevation of ROS caused by the knockdown of USP11. G Representative results of IHC staining for Ki67, USP11 and Nrf2. H The levels of mitochondrial apoptosis regulatory proteins and Nrf2/ARE signaling pathway protein, including Cleaved PARP1, Procaspase9, Cleaved Caspase9, HO 1, NQO1, Bak, Cleaved Caspase3, Cytochrome C were measured by Western Blotting.

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