Fig. 7: Nrf2 promoted the expression of USP11 at the transcriptional level.

A, B qPCR and WB analysis expression of USP11 following the transfection of Nrf2 plasmid. (n = 3). C Predicted the binding sites of Nrf2 and the promoter region of USP11 through the JASPAR database. D Dual-luciferase reporter assays were performed by co-transfection of Vector or Nrf2 and USP11 promoter reporter constructs. Nrf2 could promote the transcription of USP11. (n = 3). E Schematic of the locations of the Nrf2 binding site in the USP11 promoter. Nrf2 could bind to the first ARE site in the promoter region of USP11 through the CHIP-PCR assays. (n = 3).