Fig. 1: Upregulation of Psmb8 expression and activity in mice with I/R and patients. | Cell Death & Disease

Fig. 1: Upregulation of Psmb8 expression and activity in mice with I/R and patients.

From: Psmb8 inhibits mitochondrial fission and alleviates myocardial ischaemia/reperfusion injury by targeting Drp1 degradation

Fig. 1

A qPCR analysis of the mRNA levels of proteasomal catalytic subunits (Psmb6, Psmb7, Psmb5, Psmb9, Psmb10, and Psmb8) in the board zone of the heart (n = 6). B Immunoblot analysis of Psmb9, Psmb10, and Psmb8 protein levels in the border zone of the heart (left) and quantification of the protein levels (right, n = 4). C Neonatal rat cardiomyocytes (NRCMs) and neonatal rat cardiac fibroblasts (NRCFs) were exposed to hypoxia/reoxygenation (H/R) for 6 or 24 h. Immunoblots analysis of Psmb8 protein levels in H/R-induced NRCMs (left) and the quantification (right, n = 4). D Immunofluorescence colocalization staining of heart slices with anti-Psmb8 and α-actinin antibodies (left) and quantification of the Psmb8+ area (right, n = 6). E The fold-change of Psmb8 expression (relative to normal) in heart tissues and cardiomyocytes. The values are expressed as the mean ± SEM, and n indicates the sample number per group. *P < 0.05 and **P < 0.01 versus the sham or control group.

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