Fig. 2: Overexpression of Psmb8 in cardiomyocytes improves I/R-mediated cardiac injury and dysfunction. | Cell Death & Disease

Fig. 2: Overexpression of Psmb8 in cardiomyocytes improves I/R-mediated cardiac injury and dysfunction.

From: Psmb8 inhibits mitochondrial fission and alleviates myocardial ischaemia/reperfusion injury by targeting Drp1 degradation

Fig. 2

A Male WT mice were infected with rAAV9-Psmb8 or rAAV9-GFP control for 3 weeks and then subjected to I/R or sham surgery for 24 h. Echocardiographic examination of the left ventricle (LV) (left) and percentages of the ejection fraction (EF) and fraction shortening (FS) (right, n = 6) are shown. B Images of heart sections stained with TTC and Evans blue dye (left). Percentages of the area at risk (AAR) to the LV area or the infarct area to the LV area (right, n = 6). Bar: 2.5 mm. C Images of heart sections stained with TUNEL (red), anti-α-actinin (green) and DAPI (blue) (top, left) and the percentages of TUNEL-positive nuclei (middle, n = 6). Heart sections were stained with DHE dye (bottom, left), and ROS concentrations were quantified (right, n = 6). Bar: 50 μm. D Immunoblot analysis of Bcl-2 and Bax protein levels (left) and quantification (right, n = 4). The values are expressed as the mean ± SEM, and n indicates the sample number per group.

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