Fig. 8: Knockdown of Drp1 in Psmb8-KO mice reverses I/R-induced cardiac damage and dysfunction. | Cell Death & Disease

Fig. 8: Knockdown of Drp1 in Psmb8-KO mice reverses I/R-induced cardiac damage and dysfunction.

From: Psmb8 inhibits mitochondrial fission and alleviates myocardial ischaemia/reperfusion injury by targeting Drp1 degradation

Fig. 8

A Male WT or Psmb8-KO mice were injected with rAAV9-siCon or rAAV9-siDrp1 (1.0 × 1012 vg/mg) for three weeks and then exposed to I/R injury for another 24 h. The protein levels of Psmb8, Drp1, Mfn1 and Mfn2 were determined by immunoblotting (left), and the protein levels were quantified (right, n = 4). B Representative echocardiographic assessment of the function of the left ventricle (LV, left). The LV EF% and LV FS% were measured (left, n = 6). C The heart infarct area was examined by TTC and Evans blue staining (left). Bar: 2.5 mm. Analysis of the percentages of the area at risk (AAR) to the LV area and the infarct size to the LV area (right, n = 6). D Apoptotic cardiomyocytes in heart sections were evaluated by TUNEL (red), α-actinin (green) and DAPI (blue) staining (left). The percentages of TUNEL-positive nuclei are shown (right, n = 6). E The mRNA levels of Bax and Bcl-2 in the heart were determined by qPCR (n = 6). F Heart sections were stained with DHE dye (left), after which ROS concentrations were quantified (right, n = 6). Bar: 50 μm. The values are expressed as the mean ± SEM, and n indicates the number of samples per group.

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