Fig. 6: Ineffectiveness of EGF in protecting against glutamate-induced excitotoxicity and STSP-induced apoptosis in mouse cerebrocortical cultures. | Cell Death & Disease

Fig. 6: Ineffectiveness of EGF in protecting against glutamate-induced excitotoxicity and STSP-induced apoptosis in mouse cerebrocortical cultures.

From: An increase of lysosomes through EGF-triggered endocytosis attenuated zinc-mediated lysosomal membrane permeabilization and neuronal cell death

Fig. 6

A Microscopic images (upper) and a quantitative graph (lower) show PI-stained damaged neuronal cells after exposure to glutamate (150 µM, 12 h) with or without TPEN (1 µM), EDTA (1 mM) or EGF (100 ng/ml) in mouse cerebrocortical cultures. Scale bar: 200 µm. The bar graph indicates the fluorescence intensity of PI measured using the Image J program (mean ± SEM, n = 8 or 6 different fields taken from ≥4 independent biological replicate experiments, ####p < 0.0001 for control, and **p < 0.01 or not significant (ns) for glutamate, analyzed using ANOVA with Dunnett’s post-hoc test). B Bars indicate LDH release from damaged neuronal cells after exposure to glutamate (150 µM, 12 h) with or without TPEN (1 µM), EDTA (1 mM) or EGF (100 ng/ml) in mouse cerebrocortical cultures (mean ± SEM, n = 8 or 7 taken from ≥3 independent biological replicate experiments, ****p < 0.0001 or not significant (ns) for glutamate, analyzed using ANOVA with Dunnett’s post-hoc test). C Microscopic images (upper) and a quantitative graph (lower) show PI-stained damaged neuronal cells after exposure to staurosporine (STSP, 200 nM, 24 h) with or without zVAD (100 µM), TPEN (1 µM) or EGF (100 ng/ml) in mouse cerebrocortical cultures. Scale bar: 200 µm. The bar graph indicates the fluorescence intensity of PI (mean ± SEM, n = 6 ~ 8 different fields taken from ≥4 independent biological replicate experiments, ####p < 0.0001 for control, and ****p < 0.0001 or not significant (ns) for STSP, analyzed using ANOVA with Dunnett’s post-hoc test). D Bars indicate LDH release from damaged neuronal cells after exposure to STSP (200 nM, 24 h) with or without zVAD (100 µM), TPEN (1 µM) or EGF (100 ng/ml) in mouse cerebrocortical cultures (mean ± SEM, n = 5–8 taken from ≥4 independent biological replicate experiments, ****p < 0.0001 or not significant (ns) for STSP, analyzed using ANOVA with Dunnett’s post-hoc test).

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