Fig. 3: ZNF652 overexpression suppressed cell viability, colony formation, and cell proliferation in vitro.

A A549 and H460 cells transfected with empty control vector (Vector) or ZNF652 overexpression lentivirus (ZNF652-OE) were harvested. Expression of ZNF652 in ZNF652-OE and vector control cells were examined using RT-PCR and WB analysis. B Cell viability was measured at 0, 24, 48, and 72 h in vector control and ZNF652-OE LC cells. C Colony formation ability of vector control and ZNF652-OE LC cells were detected. Histograms of relative colony formation rates from three independent experiments. D, E EdU assay was performed to examine the proliferation of vector control and ZNF652-OE LC cells. Flow cytometry analysis of EdU staining positive cells (D). Images of EdU-stained cells and histograms of the percentage of EdU-stained cells were shown (E). Scale bars, 100 μm. F A549 and H460 cells transfected with control shRNA (shCtrl) or three different shZNF652 were detected for mRNA expression using RT-PCR. ZNF652 protein expression was detected in LC cells transfected with shZNF652-2 and shCtrl using WB analysis. G Cell viability was measured at 0, 24, 48, and 72 h in shCtrl and shZNF652-2 LC cells. H Colony formation ability of shCtrl and shZNF652-2 LC cells was detected. Histograms of relative colony formation rates from three independent experiments. I, J EdU assay was performed to examine the proliferation of shCtrl and shZNF652-2 LC cells. Flow cytometry analysis of EdU staining positive cells (I). Images of EdU-stained cells and histograms of the percentage of EdU-stained cells were shown (J). The data are presented as mean ± SD (n = 3). ns, no significant, *p < 0.05, **p < 0.01 vs. Vector or shCtrl group.