Fig. 6: ZNF652 induced LC cell cycle arrest at the G1 phase.
From: ZNF652 exerts a tumor suppressor role in lung cancer by transcriptionally downregulating cyclin D3
A Cell cycle distribution measured in vector control and ZNF652-OE A549 and H460 cells using flow cytometry assay. B p53, p21, and cyclin D1 mRNA expression were detected in vector control and ZNF652-OE LC cells using RT-PCR assay. C, D Expression of cell-cycle related protein (p53, p21, cyclin A2, cyclin E1, Cdc25A, CDK2, E2F1, cyclin D1, CDK4, and Skp2) was detected in vector control cells and ZNF652-OE LC cells using WB assay. E Cell cycle distribution measured in shCtrl and shZNF652-2 A549 and H460 cells using flow cytometry assay. F p53, p21, and cyclin D1 mRNA expression were detected in shCtrl and shZNF652-2 LC cells using RT-PCR assay. G, H. Expression of cell-cycle related protein (p53, p21, cyclin A2, cyclin E1, Cdc25A, CDK2, E2F1, cyclin D1, CDK4, and Skp2) was detected in shCtrl and shZNF652-2 LC cells using WB assay. The data are presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01 vs. Vector or shCtrl group.
